O-256 Evaluation of the fertility potential of semen using Proton NMR

Abstract Study question Is Proton NMR seminal plasma assessment a valid method for identifying biomarkers allowing to predict top-quality (TQ) embryo development? Summary answer Biomarkers important in the context of male fertility can be assessed using proton What is known already Currently, quality sperm predominantly by examining its concentration, motility, and morphology. These parameters are insufficient diagnosing infertility predicting treatment outcomes with assisted reproductive technology (ART). Metabolomics techniques have been shown potentially useful related infertility. It has proven, NMR, that there number substances semen correlate various physiological states patients may indicate impaired functioning metabolic pathways. However, relationship between development not yet investigated design, size, duration This was prospective case-control study consisted 30 samples from 13 who underwent IVF January – March 2022 at INVICTA Fertility Centre, Poland. Sperm morphology, DNA fragmentation were assessed. Before preparation fertilization, frozen perform 1H analyses, which preceded centrifugation order separate plasma. analysis results compared embryological results. Participants/materials, setting, methods possible identify pre-selected through use reference spectra quantify their concentrations internal standard addition method. Their relative (separately pairs) collated parameters, data pregnancy outcomes. A Machine Learning approach employed most variables, then used further modelling LGBM models. Main role chance allows identification measurement potential semen. The presence amino acids, organic sugar residues fatty acid derivatives confirmed applying this technique. found concentration these an individual feature - probably dependent on state organism. ratios biomarker each other more than concentrations. learning showed strong correlation several compounds obtaining TQ blastocyst. amount taurine, glutamic choline together seem factors blastocyst development. if significantly surpasses glycerophosphocholine, exceeds taurine higher citrate, or phosphorus analogue likelihood higher. would suggest pathways as Krebs cycle, one-carbon cycle metabolism modified signalling pathological conditions cannot detect. Limitations, reasons caution technique fast determination content many biomarkers, but mmol/l range. should larger group different disorders Wider implications findings rapid creation unique profiles patients, ensure personalized treatments selection highest Trial registration studies approved Ethics Committee Gdansk Regional Medical Board (No KB-3/22).